Associate Prof. Ming Li Published article on Analytical Methods with the title of " Ultrasensitive fluorometric oligonucleotide immunoassay for the simultaneous and efficient detection of two mycotoxins in agricultural products".
Abstract: An ultrasensitive fluorometric oligonucleotide immunoassay (UFOIA) based on the fluorometric oligonucleotide and the magnetic separation was proposed for the simultaneous detection of two mycotoxins. The Cy5-linked and 6-FAM-linked oligonucleotides were dissociated and applied for detecting the fluorescence signals simultaneously. The proposed UFOIA method had achieved the simultaneous and ultrasensitive detection of mycotoxins at the pg mL-1 level, which was considerably improved. This study might provide an alternative approach for detecting multi-component contaminations, which had equipped with the notable highlights of ultrasensitivity, simultaneity, simplicity, high efficiency and low background signal.
Acknowledgements: This research was funded by the National Natural Science Foundation of China (31701687), the Natural Science Foundation of Jiangsu Province (BK20170537), the Youth Talent Cultivation Program of Jiangsu University, and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD-2018-87).
DOI: https://pubs.rsc.org/en/content/articlelanding/2022/AY/D2AY00367H
