Associate Prof. Ming Li Published article on Talanta with the title of " An enhanced immunochromatography assay based on gold growth on the surface of E. coli carrier for the simultaneous detection of mycotoxins".
Abstract: An enhanced ICA strip was established and evaluated for simultaneous, semi-quantitative and quantitative detection of ochratoxin A (OTA) and aflatoxin B1 (AFB1). The signal strategy based on gold growth on the surface of E. coli K12 carrier was recommended, which was successfully self-assembled on the enhanced ICA with double test line pattern. By the visual naked eye, the semi-quantitative limit of detection (semi-Q-LOD) both reached 0.03 ng/mL for OTA and AFB1 (17-fold and 33-fold lower than conventional ICA strip). By the digitized strip reader, the quantitative LOD (Q-LOD) all identified as 0.01 ng/mL for two mycotoxins (10-fold improvement), with the detection range of 0.01-0.5 ng/mL for OTA and 0.01-0.2 ng/mL for AFB1. Furthermore, the high reliability and applicability of the enhanced ICA had been confirmed by the good correlation with the LC-MS/MS. The advantages of the improved sensitivity, high efficiency and cost saving had been reflected for the enhanced ICA.
Acknowledgements: This research was funded by the National Natural Science Foundation of China (31701687), the Natural Science Foundation of Jiangsu Province (BK20170537), the Youth Talent Cultivation Program of Jiangsu University, and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD-2018-87).
DOI: https://doi.org/10.1016/j.talanta.2022.123798
